2055 Preventing Acid Induced Enamel Demineralization Using CPP-ACP Containing Paste

Y. SAKAGUCHI, S. KATO, T. SATO, S. KARIYA, and L. CHEN, GC corpolation, Tokyo, Japan

Objectives: The objective of this in vitro study is to evaluate, by use of Quantitative Light-Induced Fluorescence (QLF), the caries preventive potential of MI Paste (MIP, GC Corp.; also known as GC Tooth Mousse (TM)), which contains 10% Casein Phosphopeptide - Amorphous Calcium Phosphate (CPP-ACP).

Methods: Bovine enamel specimens were prepared, treated with 0.3 grams of MIP and then challenged with 10 ml of a demineralization solution (0.1M Lactic acid buffer, pH 5.0) at 37 degree Celsius RH 100% for 72 hours. The enamel surface of each specimen was scanned, using QLF (Inspektor pro, Inspektor Dental Care) before and after the procedure. The collected images were then analyzed to determine the degree of demineralization. A placebo paste without CPP-ACP, a CPP-ACP free paste containing 900 ppm Fluoride and water were included as controls. The sections of enamel samples were also subjected to X-ray CT(TOSCANER-30000µhd, Toshiba) analysis. Results: QLF analysis determined that the mean change in fluorescence radiation (∆ F, %) of the MIP, the placebo paste, fluoride paste and water controls were -1.55; -9.03; - 6.98; and -16.50 respectively. Compared to the placebo and fluoride paste, MIP showed a significant protective effect against acid induced demineralization (p < 0.05). X-Ray CT further confirmed this result.

Conclusion: This QLF study confirmed the inhibitory effect of CPP-ACP on acid induced surface demineralization and demonstrated a functional role for MIP (TM) as a caries preventative agent for use in minimum intervention dentistry.