2045 Fluoride Effect on Acid Resistance Capacity of CPP-ACP Containing Material Fluoride Effect on Acid Resistance Capacity of CPP-ACP Containing Material

S. KARIYA, T. SATO, Y. SAKAGUCHI, and E. YOSHII, GC Corporation, Tokyo, Japan

Objectives: The purpose of this in vitro study was to evaluate the effect one acid resistance and neutralizing capacity when fluoride is added to a CPPACP containing material.

Methods: A CPP-ACP containing material (Tooth Mousse: TM, GC Corp.) and a second material containing 900ppm fluoride (TMP) were prepared. Bovine enamel specimens were cut out and early caries lesions were formed in acetic acid buffer solution (pH4.8, Ca: 1.5mM, P:0.9mM) for 3 days. Thirty wt.% dilution by remineralizing solution (pH7, HEPES: 20mM, Ca: 1.5mM, P: 0.9mM) was prepared for each material. Specimens were immersed in each diluted material at 37 degree Celsius for 10 days. The acid resistance of the specimen was determined by measuring the amount of calcium loss after the immersion in lactic acid buffer solution (pH4.5) for 3 hours. Neutralizing capacity of each sample was also measured by pH monitoring during acid addition. Ten µL of 1N hydrochloric acid solution was continuously added to 10mL of 10 wt.% diluted solution of TM and TMP.

Results: The amount of calcium loss of TMP and TM was 8.86µg/mm2 (SD: 1.6) and 21.7µg/mm2 (SD: 1.9) respectively and TMP showed lower levels of calcium loss compared with TM (p < 0.05). After the addition of 100µL hydrochloric acid solution, the pH of TM and TMP were reduced from 7.7 to 5.9 and 5.4 respectively. The neutralizing capacity of TM was a little superior to TMP. Fluoride has the capacity to improve the crystalline tooth structure, generation of fluoroapatite and accelerate remineralization. These effects would assist the improvement of acid resistance of TMP.

Conclusions: The acid resistance of CPP-ACP containing material was improved by the addition of fluoride.