1007 Caries Prevention Potential of a Tooth-coating Material Containing Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP)

T. SATO, K. YAMANAKA, and E. YOSHII, IADR Abstract 1007 2003GC Corporation, Tokyo, Japan

Objectives: The purpose of this study was to evaluate the caries prevention potential of a newly developed tooth coating material in vitro.

Methods: A newly developed tooth coating material: Tooth Mousse (TM, GC Corp), a CPP-ACP free placebo (PP) and fluoride (900ppm F) added placebo (FP) were prepared. Bovine enamel specimens were prepared and assigned to 6 test treatments using 10 specimens per treatment group. They were immersed in 10 wt. % diluted solution of each material for 10 minutes, followed by 10 minutes immersion in demineralization solution (pH=4.75, Ca: 0.75mM, P: 0.45mM) twice a day for 4 days. Except those treatments, the specimens were immersed in ion exchanged water (IEW) or were stayed in air of 100% RH at 37 degree Celsius. The extent of demineralization of the enamel specimens was determined by Knoop hardness measurements. The hardness reduction (∆KHN) was chosen as the primary efficacy viable. Buffer capacity of TM was also measured by pH monitoring. Ten vol. % diluted solution of TM and IEW was added to suspension of Streptococcus mutans (pH=4.5) and the pH of suspension was monitored for 2 hours.

Results: In IEW immersing group, ∆KHN of TM, FP and PP were 22.8, 51.6 and 59.6 respectively and in air staying group, ∆KHN of TM, FP and PP were 9.3, 24.2 and 30.6 respectively. Compared with FP and PP, TM showed less Knoop hardness reduction (p<0.05). Two hours after the addition of samples, the pH of TM and IEW showed 6.0 and 4.0 respectively.

Conclusions: The results show that TM is effective in preventing the enamel demineralization in vitro and has strong butter capacity to acid produced by S. mutans.