nfluence of the pretreatment of occlusal pits and fissures on the retention of a fissure sealant.

I Manhart J, Huth KC, Chen HY, Hickel R. Am J Dent. 2004 Feb;17(1):12-8. Department of Restorative Dentistry and Periodontology, Ludwig-MaximiliansUniversity, Dental School, Goethe Street 70, D-80336 Munich, Germany. manhart@manhart.com

PURPOSE: To evaluate different regimens of enamel conditioning on the retention of a pit and fissure sealant.

METHODS: 100 extracted human molars were randomized into 10 experimental groups (n = 10). Conditioning of occlusal pits and fissures was performed with either 37% H3PO4, diamond bur and 37% H3PO4, Er:YAG laser, Er:YAG laser and 37% H3PO4, air abrasion (3 different settings), air abrasion (3 different settings) and subsequent acid etching with 37% H3PO4. 24 hours after placing a compomer-based sealant, the specimens were artificially aged by thermocycling (5/55 degrees C; x1000) and immersed in basic fuchsin dye for another 24 hours. After sectioning, dye penetration along the enamel-resin-interface was determined (% of the fissure depth). Data were statistically analyzed with Kruskal-Wallis H test and Mann-Whitney U test. For qualitative investigations, two further specimens of each group were analyzed in the SEM.

RESULTS: Conventional acid etching resulted in significantly superior sealant retention than conditioning with either the Er:YAG laser or air abrasion (irrespective of particle size or air pressure). If mechanical conditioning of the laser or air abrasion was followed by acid-etching, results were statistically equal to the acid-etch only group.